ID Number		928
Study Type		In Vitro
Model		2450 MHz exposure to RBC membranes and analysis of membrane properties
Details		Rabbit erythrocytes were exposed to 2450 MHz (CW) RF for 30 minutes at 60-100 W/kg at various ambient temperatures from 15-25°C. Exposure resulted in an increase of radio-labeled 22-Na+ when at the phase transition temperature (18-22 degrees C). This increased permeability was enhanced with hypoxia as well as hyperoxia, although in sham-exposed cells there was no effect of hypoxia or hyperoxia. Further, 22-Na++ permeability was inhibited when ascorbic acid or mercaptoethanol (antioxidants) were added to the media. Use of cholesterol-enriched liposomes eliminated the phase transition as well as the MW permeability effects. When liposomes were loaded with 3-H labeled Ara-C, drug release was increased with MW exposure, and this effect was again further increased by hyperoxia and reduced with antioxidants. Exposure also resulted in a specific pattern of protein release into the media, also enhanced with hyperoxia. The protein release was also increased when EDTA was used to chelate Ca++ and Mg++, and the authors suggest a cation-bridge involved in the effect.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Univ. California Berkeley, USA
Funding Agency		Private/Instit.
Country		UNITED STATES
References		Liburdy, RP et al. Radiat. Res., (1988) 114:500-514 Liburdy, RP et al. Radiat. Res., (1987) 109:382-395 Liburdy, RP et al. Radiat. Res., (1985) 102:190-205 Liburdy, RP et al. Radiat. Res., (1985) 103:266-275 Liburdy, RP et al. Bioelectromagnetics, (1984) 5:283-291
Comments