ID Number		864
Study Type		In Vivo
Model		2450 MHz (CW) exposure to mouse bone marrow and analysis of viability
Details		Bone marrow cells from CBA x C57BL mice were exposed in vitro to 2450 MHz (CW) MW at 12 W/kg for 5-10 minutes to produce final temperatures of 37 or 45 C. Exposure to MW did not result in any structural changes in the membrane as measured by spectrofluorometric analysis of cells labelled with the napthylaminosulfone structural probe 2,4-TNS. MW exposure to achieve a final 37-degree temperature resulted in increased proliferation (determined by reconstitution of lethally irradiated mice and counting subsequent colonies in the spleen 9 days later) over cells at 37 degrees using non-RF heating in a water bath. Further, survival of lethally irradiated mice reconstituted with MW treated bone marrow cells was increased. Increased erythropoiesis was confirmed by studies showing increased iron incorporation in the spleen. MW exposure to 45 degrees resulted in decreased spleenic colony formation. When cells were incubated with the beta-receptor blocker Trimepranol (TMP) before exposure, however, the MW effects were blocked. The authors suggest that membrane beta-receptors play some role in the MW response.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Inst. Biophysics, Czech Acad Sciences, Czech Repub
Funding Agency		Private/Instit.
Country		CZECH REPUBLIC
References		Rotkovska, D et al. Acta Oncol., (1987) 26:233-236 Rotkovska, D et al. Effects of Nonionizing Electromagnetic Radiation, (1982) :64-69
Comments		The exposure conditions were not well defined, and small temperature elevations within the bone marrow cell suspension during exposure may have contributed to an increased proliferation and colony forming activity.