ID Number		844
Study Type		In Vitro
Model		2450 MHz (AM 16, 30, 90, 120 Hz) exposure to purified enzyme preparations and analysis of enzyme activity
Details		Purified rat cytochrome oxidase and adenosine triphosphatase (ATPase) were exposed to 2450 MHz (AM- 16, 30, 90, and 120 Hz) MW at an SAR of 26 W/kg for several minutes. In a similar study, glucose 6-phosphate dehydrogenase from human red blood cells and yeast, adenylate kinase from rat liver mitochondria and rabbit muscle, and rat liver microsomal NADPH cytochrome c reductase were exposed as above at SARs of up to 42 W/kg. No effects of MW exposure on enzyme activity at any modulation frequency were observed as long as the temperature was maintained at a constant temperature via cooling. In a more recent study isolated RBC membranes were exposed to 2450 MHz (CW) at 6 W/kg and ATPase activity in was assayed with the suspension maintained at various temperatures between 23-27 degrees. At the transition temperature of ~ 25 degrees, there was a decrease in Na+/K+ ATPase activity. The authors suggest that this is not due to phase transition of the lipid membrane, but probably due to temperature-induced protein-lipid interactions within the membrane and/or conformational change in the protein itself.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		EPA, RTP North Carolina, USA - allis.john@epa.gov
Funding Agency		EPA, USA
Country		UNITED STATES
References		Allis, JW et al. Bioelectromagnetics, (1987) 8:203-212 Allis, JW et al. Radio Sci., (1979) 14:85-91
Comments