ID Number		795
Study Type		In Vitro
Model		147 MHz (AM-9, 16, 60 Hz) exposure to isolated mouse B-lymphocytes and analysis of immune response
Details		Primary B-lymphocytes from ICR Swiss mice spleens were exposed to 147 MHz (AM-9, 16, or 60 Hz) for 30 minutes at ~2 W/kg and analyzed for the distribution of antigen-antibody complexes on the cell surface by immuofluorescence. Exposure did not affect Ag-Ab capping on the cell surface as long as constant temperature was maintained. Culture at 42 degrees (via non-RF heating) did, however, significantly inhibit capping. Because capping can be influenced by changes in intracelular Ca++ concentration, the study suggests that no such changes in Ca++ concentrations appear to exist in the in vitro model used for this study. In a related study, mouse spleenic lymphocytes were exposed to 2450 MHz (CW) for 30 minutes at an SAR of 45 W/kg creating culture temperatures of 37, 41, and 42.5 degrees C. Following exposure, capping was reduced in a temperature dependent manner, and the authors conclude the effects were completely due to temperature elevations.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		University of Illinois, USA
Funding Agency		Private/Instit.
Country		UNITED STATES
References		Sultan, MF et al. Bioelectromagnetics, (1983) 4:157-165 Sultan, MF et al. Bioelectromagnetics, (1983) 4:115-122
Comments		Because capping can be influenced by changes in intracelular Ca++ concentration, the study suggests that no such changes in Ca++ concentrations appear to exist in the in vitro model used for this study. As the study did include 16 Hz AM RF at 147 MHz, it also addressed the potential ELF modulation "window" phenomenon.