ID Number		78
Study Type		In Vitro
Model		450 MHz (AM) exposure to cell lines and analysis of ODC activity
Details		Reuber H35 hepatoma, Chinese hamster ovary (CHO), and 294T melanoma cell lines were exposed or sham-exposed for 1 hr to a 450 MHz microwaves (AM at 10, 16, 20, 60, and 100 Hz) at a peak field intensity of 1.0 mW/cm2 in a TEM cell. For all 3 cell lines (H35, CHO, and 294T cells) in actively growing (log-phase) cultures, a 1-hr exposure of microwaves AM at 10, 16, and 20 Hz produced a significant (up to 50%) increase in ODC activity compared to shams. ODC activity remained elevated for more than a 3-hr period in the H35 and CHO cultures, but in 294T cells it remained elevated for only 1-hr after removal of the field. Exposure to microwaves AM at 60 and 100 Hz failed to alter ODC activity. In serum starved cells, exposure did not significantly increase in ODC activity, although cells remained responsive to TPA, insulin, glucagon, and corticosteroids. The induction of ODC activity in serum starved H35 and CHO cells by the addition of 1.6 uM TPA was potentiated by microwave exposure. The authors suggest that the field may potentiate a normal growth factor receptor-mediated mechanism at the cell membrane involved in the regulation of ODC activity.
Findings		Effects
Status		Completed With Publication
Principal Investigator		University of California, Riverside, USA - craig.byus@ucr.edu
Funding Agency		DOE, USA
Country
References		Byus, CV et al. Cancer Res., (1988) 48:4222-4226
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