ID Number		681
Study Type		In Vivo
Model		UWB (PW) exposure to rats and analysis of behavioral responses
Details		CF-1 mice were exposed to UWB (PW at 102 kV/m peak amplitude, 0.97-1.03 usec pulse duration, 155-174 psec rise time, 60-600 pps) MW for 30 minutes in a GTEM cell at an SAR of up to 37 W/kg. Exposure immediately following N(G)-nitro-L-arginine methyl ester hydrochloride (L-NAME, a NOS inhibitor) reduced the L-NAME induced increase in nociception and spontaneous locomotor activity. In contrast, a similar behavioral study exposing C57BL/6 black and CF-1 mice as above for 15-45 minutes immediately following morphine injection did not result in any significant effect on morphine induced nociception or spontaneous locomotor activity responses. The authors concluded UWB pulses do not appear to affect the opioid receptor pathways as has been reported for MW in previous studies, but do seem to increase NO production. In support of this, a recent study by the authors exposed RAW 264.7 Macrophages (incubated in nitrate containing media) to UWB MW for 30 minutes at ~0.1W/kg (causing negligible temperature increase in the media). Exposure resulted in an increase in NO production in the Macrophages.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Brooks AFB, USA - ronald.seaman@brooks.af.mil
Funding Agency		AF, USA
Country		UNITED STATES
References		Seaman, RL et al. Bioelectromagnetics, (1999) 20:431-439 Seaman, RL et al. Physiol. Behav., (1998) 65:263-270
Comments		The study has no appropriate positive or heat control, no appropriate temperature monitoring of the animals during exposure, and uses a large SAR (37 W/kg) with no clear incremental doses for a dose response. The results are relevent to risk analysis, however, although they must be validated by independent lab replication or supportive evidence before they can be fully relied upon.