ID Number		530
Study Type		In Vitro
Model		42.25, 53.6, 61.2, 78.2 GHz (CW, PW) exposure to olfactory tissue in culture and analysis of membrane biochemistry
Details		Olfactory tissue extract from rats was incubated with 3H-labeled camphor for 1 hr and exposed to 900 MHz RF (AM at 0, 1, 6, 16, 32, 50, 75, or 100 Hz) at SARs from 0.5 to 18 W/kg. RF exposure decreased 3H-camphor binding in a way that was not modulation dependent and was not a linear function of SAR. Earlier studies (Bioelectromagnetics 1994, 15:183-192) reported the same RF exposures decreased binding of H3-dihydroalprenolol (DHAP) to membrane receptors of liver and olfactory mucosa cells 5-fold compared to controls, but did not affect the binding of H3-glutamate to the membrane receptors of hippocampal cells. This effect was also not dependent on the modulation or a linear effect of SAR. Since the reducing agent DTT eliminated these effects, the authors speculate oxidative stress as a mechanism. The authors suggest this may be due to shedding of receptor elements through a non-thermal enzymatic mechanism. In a subsequent study, bilayer lipid membranes (phosphatidylcholine and cholesterol in decane) were exposed to 53-78 GHz (CW & PW - square waves at square waves at rates of 1, 4, 8, 12,16, 32, 60, 100, and 1000 pulses/sec) and capacitance and conductance values measured. Both CW and PW increased membrane current in a similar fashion, and the effects were the same as with similar non-MW temperature elevations of ~1.1 degrees. Recent studies reported primary blood and cultured immune cells exposed to 42.25 GHz (CW)at power densities as low as 1.23 W/cm2 was capable of reversibly causing externalization of phosphatidylserine molecules.
Findings		Effects
Status		Completed With Publication
Principal Investigator		USSR Acad. of Science, Moscow Russia
Funding Agency		Private/Instit.
Country		RUSSIAN FEDERATION
References		Szabo, I et al. Bioelectromagnetics., (2006) 27:233-244 Alekseev, SI et al. Bioelectromagnetics, (2001) 22:288-291 Alekseev, SI et al. IEEE Trans. Biomed. Eng., (2001) 48:1013-1019 Alekseev, SI et al. Bioelectromagnetics, (2000) 21:264-271 Alekseev, SI et al. Bioelectromagnetics, (1999) 20:24-33 Alekseev, SI et al. Bioelectromagnetics, (1997) 18:89-98 Khizhnyak, EP et al. Bioelectromagnetics, (1996) 17:223-229 Alekseev, SI et al. Bioelectromagnetics, (1995) 16:124-131 Philippova, TM et al. Bioelectromagnetics, (1988) 9:347-354 Alekseev, SI et al. Biofizika, (1987) 31:290-295 Tyazhelov, VV et al. Biophysics, (1979) 23:750-751
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