ID Number		476
Study Type		In Vitro
Model		2450 MHz (CW, AM 2 Hz-20 kHz) exposure of snail ganglia neurons and analysis of signaling
Details		Snail (Helix aspersa) subesophageal ganglia neurons were exposed to 2450 MHz (CW and AM - 20% at 2 Hz-20 kHz) for 60 minutes at 13 W/kg. Exposure was initially observed to affect morphology, nucleic acid content, and a number of other parameters. In exposure to cooled neurons (8 degrees) electrical activity was inhibited, slight hyperpolarization occurred, and membrane resistance decreased. This effect was less pronounced in neurons exposed at 21 degrees, and was not observed in neurons exposed at 28 degrees. Electrical activity recovered after 60 minutes, although membrane resistance changes did not. Microwaves that were amplitude modulated produced effects that were qualitatively and directionally different than those produced by CW microwaves. Microwave exposure initially appeared to depend on Ca++, but later reports did not confirm this. The authors suggest these data indicate a physical mechanism mediated through modulatory stimulation of the neurons. Additional studies using similar exposure showed minor but consistent morphological changes in the subesophageal ganglia including broadening (dilation and clearing) of one or two of the marginal cisternae of the Golgi complexes, slight swelling of the endoplasmic reticulum, and a decrease in cytoplasmic ribosomal content. In contrast, pigmented granules showed no change in ultrastructure or calcium content. The authors conclude that these changes are consistent with a MW initiated release of bound calcium from intracellular ER stores.
Findings		Effects
Status		Completed With Publication
Principal Investigator		University of Illinois, USA
Funding Agency		Private/Instit.
Country		UNITED STATES
References		Arber, SL et al. Physiol. Chem. Phys. Med. NMR, (1986) 18:243-249 Arber, SL et al. Bioelectromagnetics, (1985) 6:257-270 Arber, SL et al. Radiat. Environ. Biophys., (1985) 24:149-156 Arber, SL et al. Physiol. Chem. Phys. Med. NMR, (1984) 16:469-475 Arber, SL et al. Physiol. Chem. Phys. Med. NMR, (1983) 15:259-260 Lin, JC et al. Physiol. Chem. Phys., (1983) 15:261-263
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