ID Number		455
Study Type		In Vitro
Model		2450 MHz (CW) exposure to rat mast cells and analysis of histamine production
Details		Peritoneal Mast cells from CD rats were isolated and exposed in vitro to 2450 MHz (CW) for 15, 30, 45, or 60 minutes (prior to and during drug administration) at SARs of 8.2 W/kg (low) or 41 W/kg (high). In other experiments, rats were exposed in vivo (dorsally) to 2450 MHz (CW) RF for 8 hours at 0.44 W/kg (low) or 2.2 W/kg (high) SAR using a horn antenna, and then Mast cells were isolated. Following isolation, Mast cells were incubated with chemicals (hypertensive compound 48/80 or the Ca++ ionophore A-23187) or immunological stimulating agents (ConA) to produce a histamine response. In vitro exposures were performed both prior to and during drug incubation, while in vivo exposures were performed in the rat with drug incubation taking place on isolated Mast cell cultures immediately following isolation. No effects of microwave exposure (either in vivo or in vitro) were observed on Mast cell viability, toluidine blue metachromasia, histamine content, and size of peritoneal mast cells. There were no effects of exposure on histamine secretion following chemical challenge, although effects due to elevated temperatures were observed. Finally, exposure of rats to microwaves did not affect blood pressure either before or after treatment with the hypertensive drug 48/80. The authors conclude that neither immunological nor calcium transport pathways for histamine secretion in Mast cells are affected by 2450 MHz (CW) exposure.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		NIEHS, RTP North Carolina, USA
Funding Agency		NIEHS, USA
Country		UNITED STATES
References		Galvin, MJ et al. Int. J. Radiat. Biol., (1981) 39:671-675 Ortner, MJ et al. Cell Biophys, (1980) 2:127-138
Comments