ID Number		39
Study Type		In Vivo
Model		2450 MHz (CW&PW) exposure to rats and analysis of DNA breaks in brain cells
Details		Sprague Dawley (male) rats were exposed to 2450 MHz (CW & PW-2 usec pulses, 500 pulses per second) at 0.6 and 1.2 W/kg for 2 hours + 4 hour recovery in a circular waveguide exposure system. Exposure had no effect on DNA single or double strand breaks using both neutral and alkaline Olive (1992, Exp. Cell Res. 198:259-267) or Singh comet assay methods. All samples were run double blind. Significant DNA damage was detected using the Olive comet assay following 4 Gy gamma irradiation, and a high degree of variability was observed using the Singh comet method. In addition, for each comet assay technique a set of slides was treated for 2 hrs with proteinase K (1 mg/ml at 37oC), although PK treatment did not increase the sensitivity of either comet assay.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		Washington University, USA - rotiroti@radonc.wustl.edu
Funding Agency		Motorola
Country		UNITED STATES
References		Malyapa, RS et al. Radiation Research., (1998) 149:396-400 Malyapa, RS et al. Radiat. Res., (1998) 149:637-645
Comments		These studies address the Lai & Singh reports of DNA single and double strand breaks in rat brain cells in vivo in response to 2450 MHz CW & PW exposure (Bioelectro-magnetics (1995) 16:207-210; Int J Radiat Biol (1996) 69:513-521), as well as the potential differences between the Olive and the Singh comet assay methods.