ID Number		385
Study Type		In Vitro
Model		100 Hz  250 MHz (CW), 900, 1800 MHz (CW) exposure of cell lines and plant cells and analysis of growth and viability
Details		L929- and 3T3 mouse fibroblasts , plant protoplasts isolated from tissue of larch and pollen grains collected from hazel were exposed to 100 Hz up to 250 MHz (0,1 up to 100 kV/m) in microelectrode chambers (duration of exposure: seconds up to several weeks) and 900 MHz and 1,8 GHz (several 10 kV/m possible) in slot line resonators (preliminary test exposures of mouse fibroblasts). Diverse parameters such as increase of cell volume, proliferation rate, metabolic activity, adhesion behaviour, membrane permeability, changes of the cytoskeleton etc. were investigated. At the technical micro structures local heating, electrical goodness, rotation rate and leviation (accentuation) of particles for specific field strength calculations were investigated. Cell experiments in the range of 100 Hz to 250 MHz resulted in definite key data where the cells were not damaged, potentially damaged or actually damaged; temperature effects could be clearly differentiated from field effects. Field parameters near the cells were very good determinable. Microstructures for the exposure of living cells at 900 MHz and 1,8 GHz were developed and successfully tested. The authors conclude that high field strength RF can be used to manipulate and position cells in culture without significant damage. Start date July 1997, end date August 1999
Findings		No Effects
Status		Completed With Publication
Principal Investigator		University of Berlin, FRG
Funding Agency		FGF, Germany
Country		GERMANY
References		Fuhr, G et al. Hum Reprod, (1998) 13:136-141
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