ID Number		326
Study Type		In Vitro
Model		1.5 GHz (PDC), 2.1 GHz (UMTS, CW), 2.4 GHz (CW) exposure to MO54 and CHO-K1 cells and analysis of DNA damage
Details		MO54 and CHO-K1 cells were exposed to 2450 MHz (CW, PW) RF at high SARs (5 to 200 W/kg). In an initial study, exposure for 2 days at up to 50 W/kg in a circular waveguide +/- co-treatment with bleomycin had no effect on HGPRT locus mutations, and exposure at up to 100 W/kg had no effect on DNA strand breaks using an alkaline comet assay. In the 2005 study, human malignant glioma MO54 cells were exposed to 2450 MHz for 2 hrs at SARs of 5, 10, 20, 50, 100 and 200W/kg. Thermal controls were performed at 39, 41 and 44 ºC. The authors report no significant effects on DNA damage as measured by tail moment, tail length and tail percent in comet assays at SARs up to 200 W/kg, although effects were observed using bleomycin (positive control) treatment. In a 2006 publication, the authors reported a similar lack of effects in human glioblastoma A172 cells and normal human IMR-90 fibroblasts exposed to 2.1 GHz (UMTS, CW) RF at up to 0.8 W/kg for 2 or 24 h (~simulating base station exposure levels as defined by the ICNIRP whole body limits).
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Hirosaki University, Japan - miyskosh@cc.hirosaki-u.ac.jp
Funding Agency		ARIB, Japan, MIC, Japan
Country		JAPAN
References		Sakuma, N et al. Bioelectromagnetics, (2006) 27:51-57 Miyakoshi, M et al. Electricial Engineering in Japan, (2002) 141:9-15
Comments		The authors report temperature of the medium increased to 39 degrees C after a 2-hour exposure at 50 W/kg and 41 degrees C after 2-hour exposure to 100 W/kg. The findings are in line with those of Maes et al. and Vijayalaxmi et al. (no increase in chromosomal aberrations under similar exposure conditions) and in contrast to Lai and Singh who reported differential response of cultured mammalian cells to CW and PW RF.