ID Number		2822
Study Type		In Vitro
Model		Review of mechanisms of microwave effects and investigation of the potential cytotoxicity of RF exposure on central nervous system by exposing rat pheochromocytoma (PC12) cells in vitro to 2.856 GHz (4 W/kg) for 8 h a day for 2 days.
Details		AUTHORS' ABSTRACT: Zhou et al. 2019 (IEEE #7215): To investigate the potential cytotoxicity of radiofrequency (RF) radiation on central nervous system, rat pheochromocytoma (PC12) cells were exposed to 2.856 GHz RF radiation at a specific absorption rate (SAR) of 4 W/kg for 8 h a day for 2 days in 35 mm Petri dishes. During exposure, the real-time variation of the culture medium temperature was monitored in the first hour. Reactive oxygen species (ROS) production, intracellular Ca2+ concentration, and cell apoptosis rate were assessed immediately after exposure by flow cytometry. The results showed that the medium temperature raised about 0.93 °C, but no significant changes were observed in apoptosis, ROS levels or intracellular Ca2+ concentration after treatment. Although several studies suggested that RF radiation does indeed cause neurological effects, this study presented inconsistent results, indicating that 2.856 GHz RF radiation exposure at a SAR of 4 W/kg does not have a dramatic impact on PC12 cells, and suggests the need for further investigation on the key cellular endpoints of other nerve cells after exposure to RF radiation.
Findings		No Effects
Status		Completed With Publication
Principal Investigator
Funding Agency		?????
Country		CHINA
References		Zhou, H et al. Electromagnetic Biology and Medicine., (2019) 38:102-110 Zhao, X et al. IEEE Microw Wireless Compon Lett., (2020) 30:1205-1208 Zhao, X et al. Int. J. Radiat. Res., (2021) 19:483-494
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