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EMF Study
(Database last updated on Mar 27, 2024)

ID Number 2810
Study Type In Vitro
Model The aim of these studies was to investigate the effect (apoptosis, oxidative stress, etc) of Se and Zn on RF (1 h] exposed cells in culture.
Details

AUTHORS' ABSTRACT: Ozsobaci et al. 2018 (IEEE #7154): There is a widespread use of 2.4/GHz electromagnetic radiation emitting devices especially in communication and education. Recent studies show the adverse effects of electromagnetic fields (EMF) such as oxidative stress, cellular damage and apoptosis on tissues. Selenium (Se) has an antioxidant properties by inhibiting oxidative damage being within the structure of antioxidant enzymes like glutathione peroxidase (GSH-Px) and it has also regulatory function for cell cycle and apoptosis. The aim of this study was to investigate the effect of Se on 2.4/GHz frequency EMF exposed human embryonic kidney cells (HEK293) by means of alterations in apoptotic and oxidative stress parameters. Our study was planned as control, EMF, 100/nM Se/+/EMF, 200/nM Se/+/EMF groups. EMF groups were exposed to 2.4/GHz EMF for 1/h, element groups were incubated with two different doses of Se added cell culture medium for 48/h before EMF exposure. MDA levels were significantly higher whereas SOD and GSH-Px activities were significantly lower in EMF compared to control. 100 and 200/nM Se/+/EMF application decreased MDA levels, increased SOD and GSH-Px activities than EMF. Apoptosis and caspase-3 were statistically significantly higher but bcl-2 was lower in EMF than control. Apoptosis and caspase-3 were lower in 100 and 200/nM Se/+/EMF, although bcl-2 were higher than EMF. In conclusion, Se has protective effects against 2.4/GHz EMF-induced oxidative stress by reducing lipid peroxidation, regulating SOD and GSH-Px activity. Also, Se has inhibitory effect on 2.4/GHz EMF induced apoptosis by increasing the expression of anti-apoptotic protein bcl-2 and suppressing apoptosis regulatory protein caspase-3. AUTHORS' ABSTRACT: Ozsobaci et al. 2019 (IEEE #7310): Several epidemiological studies have shown that exposure to electromagnetic radiation (EMR) can be harmful to human health. The purpose of this study was to examine oxidative parameters and apoptosis induced by EMR in human kidney embryonic cells (HEK293) and to investigate whether zinc (Zn) has protective effect on EMR-induced apoptosis in HEK293 cells. For our experiment, HEK293 cells were divided into four main groups, control, EMR, 50 ¼M Zn + EMR, and 100 ¼M Zn + EMR. HEK293 cells of EMR groups were exposed to 2.45 GHz EMR for 1 h. In Zn groups, HEK293 cells were incubated with different concentrations of Zn for 48 h before EMR exposure. Oxidative stress parameters were determined by spectrophotometric method; bcl-2 and caspase-3 were assessed immunohistochemically and TUNEL method was performed for apoptotic activity. EMR group had higher malondialdehyde (MDA) level and lower superoxide dismutase (SOD) activity compared with control group. In Zn-applied groups, MDA was decreased and SOD activity was increased compared with EMR group. The number of the apoptotic cells and caspase-3 immunopositive cells at EMR group was increased significantly compared with the control group, whereas bcl-2 was decreased. Besides, Zn-treated groups showed a significant reduction in the number of apoptotic cells and caspase-3 from that of EMR group, whereas there was an increase in bcl-2 immunopositivity. Our findings show that EMR caused oxidative stress and apoptotic activation in HEK293 cells. Zn seems to have protective effects on the EMR by increasing SOD activity and bcl-2 immunopositivity, decreasing lipid peroxidation and caspas-3 immunopositivity.

Findings Effects
Status Completed With Publication
Principal Investigator Istanbul Univ, Istanbul, Turkey.
Funding Agency ?????
Country TURKEY
References
  • Pastac Ozsobaci, N et al. J Trace Elem Med Biol., (2018) 50:572-579
  • Ozsobaci, NP et al. Biological Trace Element Research. , (2020) 194:368-378
  • Duzgun Ergun, D et al. Electromagn Biol Med., (2022) 41:93-100
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