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Rat astrocyte (II) cells were exposed to 1.8 GHz (GSM-like) RF for 48 hours in a waveguide beginning at heat generating power levels and successively decreasing field strengths to produce athermal exposures. A dose response curve was constructed for membrane ion (Ca++) permeability and resting membrane potential. A similar dose response curve was constructed using conventional (non-RF) heating. Initial results indicated non-thermal, threshold, and window effects on Ca++ permeability due to RF exposure. Subsequent studies (Wojtysiak et al, 22nd Bioelectromagnetics Meeting, Munich, 2000) did not reveal differences in K+ permeability using patch clamp techniques. Potassium channels were also immunostained (Kullnich et al, 22nd Bioelectromagnetics Meeting, Munich, 2000) using an antibody to protein Kv1.6, and fluorescence determined using a microscope digital imaging system, and no significant effects of RF were observed. However, these findings were never published in the peer reviewed literature. |