ID Number		2756
Study Type		In Vitro
Model		Mouse Leydig (TM3) cells were exposed in vitro (1950 MHz, 24 h, 3 W/kg) and at 0, 1, 2, 3, 4, and 5 days after exposure, cell proliferation, percentage of apoptosis, cellular reactive oxygen species (ROS), and testosterone level was measured.
Details		AUTHORS' ABSTRACT: Lin et al. 2017 (IEEE #6904): More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Fourth Military Med Univ, Xian, China
Funding Agency		?????
Country		CHINA
References		Lin, YY et al. Int J Environ Res Public Health., (2017) 15:17-
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