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EMF Study
(Database last updated on Mar 27, 2024)
ID Number |
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2741 |
Study Type |
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In Vivo |
Model |
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To investigate the effect of a modifified Wuzi Yanzong Pill (WZYZP) on the male rats' testis after microwave radiation, as well as its potential mechanism. |
Details |
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AUTHORS' ABSTRACT: Hu et al. 2017 (IEEE #6862): Objective: To investigate the effect of a modififi ed Wuzi Yanzong Pill (”PM—8, WZYZP) on the
male rats' testis after microwave radiation, as well as its potential mechanism. Methods: Forty-fifi ve male rats were
randomly assigned to three groups: the control group, the radiation group, and the WZYZP group. The rats in the
radiation group and WZYZP group were exposed to microwave radiation for 15 min once, while the rats in the
control group were not exposed to any radiation. The rats in the WZYZP group were given a modififi ed of WZYZP
by gavage daily for 7 days. Apoptosis in the testis was evaluated using terminal-deoxynucleoitidyl transferase
mediated nick end labeling (TUNEL) assay. Histopathological alterations of the testis were observed by
haematoxylin-eosin (HE) staining. Tat-interactive protein, 60kD (Tip60) and p53 expressions were determined by
Western blotting. Results: The apoptosis index (AI) in the radiation group was higher than that of the WZYZP
group and control group on day 1 (D1), day 7 (D7) day 14 (D14) after radiation (P<0.05). The seminiferous
tubules were of normal morphology in the control group. In the radiation group, the partial seminiferous
tubules were collapsed, basement membranes of the seminiferous epithelia became detached. WZYZP could
restore the morphological changes. There was no expression of Tip60 among the three groups on D7 and
D14. The expression of p53 was higher in the radiation group than in the control group (P<0.05). WZYZP
could down-regulate the rising p53 induced by radiation on D7 and D14 (P<0.05). Conclusion: A modified
WZYZP may affect germ cells, and its protective effects may partly result from its ability to intervene in Tip60
mediated apoptosis. |
Findings |
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Effects |
Status |
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Completed With Publication |
Principal Investigator |
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Air Force General Hospital, PLA, Beijing, China
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Funding Agency |
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?????
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Country |
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CHINA |
References |
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Hu, HX et al. Chin J Integr Med. , (2017) :-
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Comments |
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