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(Database last updated on Mar 27, 2024)
| ID Number | 274 | |
| Study Type | In Vitro | |
| Model | 835 MHz (CDMA, FM), 2450 MHz (CW) exposure to cell lines and human lymphocytes and analysis of micronuclei and chromosome aberrations | |
| Details | Human lymphocytes were exposed to 2450 MHz (CW) RF either continually or intermittent (30-min on/30-min off - protocol repeated 3 times) for a total exposure time of 90 minutes at SARs in up to 47.05 W/kg. In one set of studies, the majority of the cells were described as being exposed to SARs greater than 6.53 W/kg. In a second study performed in collaboration with the Roti Roti group at Washington University in St. Louis, human lymphocytes were exposed at an average SAR of ~ 12 W/kg. Neither study showed an effect of RF on the incidence of micronuclei. Related studies from the same group exposed human peripheral lymphocytes to 835.62 MHz RF for 24 hours in an RTL exposure system at an average SAR of 4.4 or 5.0 W/kg. Immediately after the 24 hour exposure, lymphocytes were stimulated with mitogen and cultured for 48 or 72 hours to determine the extent of genetic damage. Exposure to 150 cGy gamma radiation served as a positive control. RF exposure did not affect mitotic indices, incidence of exchange aberrations, excess fragments, binucleate cells or micronuclei formation. RF also had no effect on growth rate or mitotic index. A subsequent study exposing primary human lymphocytes to 2450 MHz and 8.2 GHz (PW) at either 2.13 or 20.71 W/kg, respectively, for 2 hours also revealed no significant change in either micronuclei, chromosome aberrations, or proliferation in the presence of immunostimulatory agents. AUTHORS' ABSTRACT: Vijayalaxmi et al. 2013 (IEEE #5307): Peripheral blood samples from four healthy volunteers were collected and aliquots were exposed in vitro for 2 h to either (i) modulated (wideband code division multiple access, WCDMA) or unmodulated continuous wave (CW) 2450 MHz radiofrequency (RF) fields at an average specific absorption rate of 10.9 W/kg or (ii) sham-exposed. Aliquots of the same samples that were exposed in vitro to an acute dose of 1.5 Gy ionizing gamma-radiation (GR) were used as positive controls. Half of the aliquots were treated with melatonin (Mel) to investigate if such treatment offers protection to the cells from the genetic damage, if any, induced by RF and GR. The cells in all samples were cultured for 72 h and the lymphocytes were examined to determine the extent of genetic damage assessed from the incidence of micronuclei (MN). The results indicated the following: (i) the incidence of MN was similar in incubator controls, and those exposed to RF/sham and Mel alone; (ii) there were no significant differences between WCDMA and CW RF exposures; (iii) positive control cells exposed to GR alone exhibited significantly increased MN; and (iv) Mel treatment had no effect on cells exposed to RF and sham, while such treatment significantly reduced the frequency of MN in GR-exposed cells. |
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| Findings | No Effects | |
| Status | Completed With Publication | |
| Principal Investigator | University of Texas, USA - vijay@uthscsa.edu | |
| Funding Agency | AF, USA | |
| Country | UNITED STATES | |
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