ID Number		2686
Study Type		In Vitro
Model		Investigation of effects of GSM 900 MHz on growth, proliferation and morphology of stem, adipose and cancer cells in culture.
Details		AUTHORS' ABSTRACT: Shahbazi-Gahrouei et al. 2016 (IEEE #6667): Background: As the use of mobile phones is increasing, public concern about the harmful effects of radiation emitted by these devices is also growing. In addition, protection questions and biological effects are among growing concerns which have remained largely unanswered. Stem cells are useful models to assess the effects of radiofrequency electromagnetic fields (RF-EMF) on other cell lines. Stem cells are undifferentiated biological cells that can differentiate into specialized cells. Adipose tissue represents an abundant and accessible source of adult stem cells. The aim of this study is to investigate the effects of GSM 900 MHz on growth and proliferation of mesenchymal stem cells derived from adipose tissue within the specific distance and intensity. Materials and Methods: ADSCs were exposed to GSM mobile phones 900 MHz with intensity of 354.6 ¼W/cm2 square waves (217 Hz pulse frequency, 50% duty cycle), during different exposure times ranging from 6 to 21 min/day for 5 days at 20 cm distance from the antenna. MTT assay was used to determine the growth and metabolism of cells and trypan blue test was also done for cell viability. Statistical analyses were carried out using analysis of one way ANOVA. P<0.05 was considered to be statistically significant. Results: The proliferation rates of human ADSCs in all exposure groups were significantly lower than control groups (P<0.05) except in the group of 6 minutes/day which did not show any significant difference with control groups. Conclusion: The results show that 900 MHz RF signal radiation from antenna can reduce cell viability and proliferation rates of human ADSCs regarding the duration of exposure. AUTHORS' ABSTRACT: Shahbazi-Gahrouei et al. 2018 (IEEE #7092): Background: Today, using cellular phone and its harmful effects in human life is growing. The aim of this study is to investigate the effect of the global system for mobile communication (GSM) 900 MHz cellular phone radiofrequency waves on growth, morphology, and proliferation rate of mesenchymal stem cells and Michigan Cancer Foundation (MCF-7) cells within the specific distance and intensity. Methods: MCF-7 and human adipose-derived stem cells (HADSCs) were exposed to GSM cellular phones 900 MHz frequency with intensity of 354.6 ¼W/cm2 during different exposure times 6, 21, 51, and 101 min/day with an interval of 10 min for each subsequent radiation exposure for 3 and 5 days at 10 and 20 cm distances from antenna. 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay and trypan blue test were used to determine the growth of cells and cell viability, respectively. Statistical analyses were carried out using three-way ANOVA. Differences were significant when P < 0.05. Results: The proliferation rates of both MCF-7 and HADSCs cells in all exposure groups were significantly lower than controls (P < 0.05). There was a significant effect on the percentage of cell survival with increase the period of time from 3 to 5 days for MCF-7 (P < 0.01) and HADSCs (P = 0.02), respectively. Variations in distance had no significant effect on the percentage of cell survival (P = 0.35) on MCF-7 (P = 0.02) and HADSCs (P = 0.09) cells, respectively. Conclusions: The results showed that radiation of GSM 900 MHz cellular phone may be reduced cell viability and proliferation rates of both cells. It is recommended to reduce exposure time, increase distance from antenna, and reserve the use of cell phones for shorter conversations to prevent its biological and harmful effects. Further studies with other intensities and frequencies on different cells are recommended.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Isfahan U of Med Sciences, Isfahan, Iran
Funding Agency		Iran, Isfahan U of Med Sciences
Country		IRAN (ISLAMIC REPUBLIC OF)
References		Shahbazi-Gahrouei, D et al. J Biomed Phys Eng., (2016) 6:243-252 Shahbazi-Gahrouei, D et al. Int J Prev Med. , (2018) 9:51-
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