ID Number		268
Study Type		In Vitro
Model		2450 MHz (CW) exposure to cell lines and analysis of DNA integrity
Details		C3H 10T1/2 fibroblast cells were exposed to 2450 MHz RF for 2 hours at 1.9 W/kg or Cis-Platinum (CDDP), followed by exposure or sham exposure to 4 Gy of gamma radiation (137Cs). Cells were then analyzed for DNA breaks using the Olive comet assay (1992, Exp. Cell Res. 198:259-267). While gamma rays alone induced the predicted level of DNA damage, no DNA damage was detected after exposure to 2450 MHz or CDDP alone. CDDP could significantly reduce both tail length and normalized comet moment in 4 GY treated cells, although 2450 MHz RF exposure could not, indicating no crosslinking had occured in 2450 MHz RF exposed cells. When control samples were treated with proteinase K, DNA migration did increase, although detection of 4 Gy initiated DNA breaks was more sensitive in the absence of proteinase K. This study addresses comments made by Drs. Lai and Singh that the Olive comet assay may not be appropriate to observe DNA breaks as originally reported in 1994 (Lai & Singh, Int J Radiat Biol (1996) 69(4):513-521 & Bioelectromagnetics (1995) 16(3):207-210) due to the absense of a proteinase K digestion step that may cause a lack of detection of DNA breaks due to 2450 MHz RF induced DNA-DNA and DNA-protein crosslinking.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		Washington University, St. Louis MO, USA
Funding Agency		Motorola
Country		UNITED STATES
References		LaGroye, I et al. Radiation Research, (2004) 161:201-214
Comments