ID Number		2648
Study Type		In Vivo
Model		The aim of present work is to elucidate the nature of the metabolic messenger through which physiological solution (PS) treated by non-thermal (NT) microwaves (MW) could modulate heart muscle hydration of rats.
Details		AUTHOR'S ABSTRACT: Ayrapetyan 2015 (IEEE #6154): The weak knowledge on the nature of cellular and molecular mechanisms of biological effects of NIR such as static magnetic field, infrasound frequency of mechanical vibration, extremely low frequency of electromagnetic fields and microwave serves as a main barrier for adequate dosimetry from the point of Public Health. The difficulty lies in the fact that the biological effects of NIR depend not only on their thermodynamic characteristics but also on their frequency and intensity "windows", chemical and physical composition of the surrounding medium, as well as on the initial metabolic state of the organism. Therefore, only biomarker can be used for adequate estimation of biological effect of NIR on organisms. Because of the absence of such biomarker(s), organizations having the mission to monitor hazardous effects of NIR traditionally base their instruction on thermodynamic characteristics of NIR. Based on the high sensitivity to NIR of both aqua medium structure and cell hydration, it is suggested that cell bathing medium is one of the primary targets and cell hydration is a biomarker for NIR effects on cells and organisms. The purpose of this article is to present a short review of literature and our own experimental data on the effects of NIR on plants' seeds germination, microbe growth and development, snail neurons and heart muscle, rat's brain and heart tissues. AUTHORS' ABSTRACT: Narinyan and Ayrapetyan 2016 (IEEE #6540 ): Previously, we have suggested that cell hydration is a universal and extra-sensitive sensor for the structural changes of cell aqua medium caused by the impact of weak chemical and physical factors. The aim of present work is to elucidate the nature of the metabolic messenger through which physiological solution (PS) treated by non-thermal (NT) microwaves (MW) could modulate heart muscle hydration of rats. For this purpose, the effects of NT MWtreated PS on heart muscle hydration, [3H]-ouabain binding with cell membrane, 45Ca2+ uptake and intracellular cyclic nucleotides contents in vivo and in vitro experiments were studied. It is shown that intraperitoneal injections of both Sham-treated PS and NT MWtreated PS elevate heart muscle hydration. However, the effect of NT MWtreated PS on muscle hydration is more pronounced than the effect of Sham-treated PS. In vitro experiments NT MWtreated PS has dehydration effect on muscle, which is not changed by decreasing Na+ gradients on membrane. Intraperitoneal injection of Sham- and NT MWtreated PS containing 45Ca2+ have similar dehydration effect on muscle, while NT MWtreated PS has activation effect on Na+/Ca2+ exchange in reverse mode. The intraperitoneal injection of NT MWtreated PS depresses [3H]-ouabain binding with its high-affinity membrane receptors, elevates intracellular cAMP and decreases cGMP contents. Based on the obtained data, it is suggested that cAMP-dependent signaling system serves as a primary metabolic target for NT MW effect on heart muscle hydration.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Life Sciences Int Postgrad Educational Center, UN
Funding Agency		?????, (vacant)
Country		ARMENIA
References		Ayrapetyan, S Electromagn Biol Med., (2015) 34:197-210 Narinyan, L et al. Electromagnetic Biology and Medicine., (2017) 36:182-191 Deghoyan, A et al. Electromagn. Biol. Med., (2012) 31:132-142
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