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EMF Study
(Database last updated on Mar 27, 2024)
ID Number |
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2595 |
Study Type |
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In Vitro |
Model |
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Effects of 900-MHz exposure on gene sequences of the yeast Candida albicans and on rats infected with the yeast. |
Details |
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AUTHORS' ABSTRACT: Shahin-jafari et al. (IEEE #6376): Over the last decade, communication industries have witnessed a tremendous expansion,
while, the biological effects of electromagnetic waves have not been fully elucidated. Current study aimed at evaluating the mutagenic effect of long-term exposure to 900-MHz radiation on alpha-Int1 gene sequences of Candida albicans. A standard 900 MHz radiation generator was used for radiation. 10 ml volumes from a stock suspension of C. albicans were transferred into 10 polystyrene tubes. Five tubes were exposed at 4 C to a fixed magnitude of radiation with different time periods of 10, 70, 210, 350 and 490 h. The other 5 tubes were kept far enough from radiation. The samples underwent genomic DNA extraction. PCR amplification of alpha-Int1 gene sequence was done using one set of primers. PCR products were resolved using agarose gel electrophoresis and the nucleotide sequences were determined. All samples showed a clear electrophoretic band around 441 bp and further sequencing revealed the amplified DNA segments are related to alpha-Int1 gene of the yeast. No mutations in the gene were seen in radiation exposed samples. Long-term exposure of the yeast to mobile phone radiation under the above mentioned conditions had no mutagenic effect on alpha-Int1 gene sequence.
AUTHORS' ABSTRACT: Bayat et al. 2017 (IEEE #7213): Mobile phones communicate with base stations using 900 MHz microwaves. The current
study was aimed to survey the effects of long-term 900 MHz microwave exposure of mice on experimentally
induced cutaneous candidiasis. Forty inbred, male, BALB/c mice were randomly divided
into four groups. Cutaneous lesions with Candida albicans were experimentally induced on the
lateral-back skin of the 20 mice. One group of the diseased mice were exposed (6 h per day and
7 d per week) to 900 MHz microwave radiation, while the other groups were not exposed. Two
unexposed control groups were also included. The skin lesions were regularly monitored and the
live candida cell density was enumerated using the colony-forming unit (CFU) assay. The process
was repeated after a one week resting interval. One week later, all mice were challenged through
intra tail veins using LD90 dose of C. albicans. Mortality of the mice was recorded and the candida
load of the kidney homogenates from died animals was counted. 900 MHz microwave exposed mice
had 1.5 day and 3.7 day delays on wound healing in stages two. Live Candida inoculated Wave
exposed (LCW) mice also showed higher yeast loads in skin lesions at days 5, 7 and 9 post inoculation.
Survival analysis of live candida challenged mice showed the radiation exposed group is
prone to death induced by systemic infection and candida enumeration from the kidney homogenates
showed radiation exposed animals have had significantly higher yeast load in the tissue. In
collection, long-term 900 MHz radiation exposure of mice led to longevity of skin wounds and susceptibility of the animals to systemic challenge and higher incidences of microorganisms in internal tissues. |
Findings |
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No Effects |
Status |
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Completed With Publication |
Principal Investigator |
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Islamic Azad U, Tehran, Iran
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Funding Agency |
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Iran, Islamic Azah University
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Country |
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IRAN (ISLAMIC REPUBLIC OF) |
References |
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Shahin-jafari, A et al. Saudi Journal of Biological Sciences., (2016) 23:426-433
Bayat, M et al. Saudi Journal of Biological Sciences., (2017) 24:907-914
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Comments |
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