ID Number		2593
Study Type		In Vitro
Model		Cell survival, intracellular calcium and reactive oxygen species (ROS) levels were measured in HMO6 cells cultured for 4 h under oxygen-glucose deprivation with or without exposure to EMF 10, 50, or 100 Hz/1 mT and 50 Hz/0.01, 0.1, or 1 mT).
Details		AUTHORS' ABSTRACT: Duong and Kim 2016 (IEEE #6374): Purpose The aim of this research was to demonstrate the protective effects of electromagnetic field (EMF) exposure on the human microglial cell line, HMO6, against ischemic cell death induced by in vitro oxygen-glucose deprivation (OGD). Materials and methods HMO6 cells were cultured for 4 h under OGD with or without exposure to EMF with different combinations of frequencies and intensities (10, 50, or 100 Hz/1 mT and 50 Hz/0.01, 0.1, or 1 mT). Cell survival, intracellular calcium and reactive oxygen species (ROS) levels were measured. Results OGD caused significant HMO6 cell death as well as elevation of intracellular Ca(2+) and ROS levels. Among different combinations of EMF frequencies and intensities, 50 Hz/1 mT EMF was the most potent to attenuate OGD-induced cell death and intracellular Ca(2+) and ROS levels. A significant but less potent protective effect was also found at 10 Hz/1 mT, whereas no protective effect was found at other combinations of EMF. A xanthine oxidase inhibitor reversed OGD-induced ROS production and cell death, while NADPH oxidase and mitochondrial respiration chain complex II inhibitors did not affect cell death. Conclusions 50 Hz/1 mT EMF protects human microglial cells from OGD-induced cell death by interfering with OGD-induced elevation of intracellular Ca(2+) and ROS levels, and xanthine oxidase is one of the main mediators involved in OGD-induced HMO6 cell death. Non-invasive treatment of EMF radiation may be clinically useful to attenuate hypoxic-ischemic brain injury.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Gachon U , Seongnam , Kyeonggi-Do , Korea
Funding Agency		?????
Country		KOREA, REPUBLIC OF
References		Duong, CN et al. Int J Radiat Biol., (2016) 92:195-201
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