ID Number		2537
Study Type		In Vitro
Model		Heat shock protein (HSP) expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity were analyzed in human neuroblastoma SH-SY5Y cells exposed for 2 h and 4 h to 1800 MHz.
Details		AUTHORS' ABSTRACT: Calabro et al. 2012 (IEEE 6218): AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working. METHODS: Neuron-like cells, obtained by retinoic-acid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands. RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure. CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.
Findings		Effects
Status		Completed With Publication
Principal Investigator		University of Messina, 98166 Messina, Italy.
Funding Agency		?????
Country		ITALY
References		Calabro, E et al. World J Biol Chem., (2012) 3:34-40
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