ID Number		2534
Study Type		In Vitro
Model		The fluorescent dye DCFH was used in flow cytometry to detect intracellular reactive oxygen species (ROS) in human peripheral blood mononuclear cells exposed to 900 MHz (GSM) at 0.4 W/kg for 1, 2, 4, 6, and 8 h.
Details		AUTHORS' ABSTRACT: Lu et al. 2012 (IEEE #6214): We demonstrate that reactive oxygen species (ROS) plays an important role in the process of apoptosis in human peripheral blood mononuclear cell (PBMC) which is induced by the radiation of 900MHz radiofrequency electromagnetic field (RFEMF) at a specific absorption rate (SAR) of <0.4W/kg when the exposure lasts longer than two hours. The apoptosis is induced through the mitochondrial pathway and mediated by activating ROS and caspase-3, and decreasing the mitochondrial potential. The activation of ROS is triggered by the conformation disturbance of lipids, protein, and DNA induced by the exposure of GSM RFEMF. Although human PBMC was found to have a self-protection mechanism of releasing carotenoid in response to oxidative stress to lessen the further increase of ROS, the imbalance between the antioxidant defenses and ROS formation still results in an increase of cell death with the exposure time and can cause about 37% human PBMC death in eight hours.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Jinan University, Guangzhou, China.
Funding Agency		?????
Country		CHINA
References		Lu, YS et al. Oxidative Medicine and Cellular Longevity., (2012) 2012:Article ID 740280-(8 pages)
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