ID Number		2517
Study Type		Human / Provocation
Model		Blood samples from 45 patients were obtained before and after contrast agent-enhanced cardiac MR imaging and lymphocytes were analyzed for double-strand breaks (DSBs).
Details		AUTHORS' ABSTRACT: Brand et al. 2015 (IEEE #6157): Purpose To evaluate the ability of magnetic resonance (MR) imaging to induce deoxyribonucleic acid (DNA) damage in patients who underwent cardiac MR imaging in daily routine by using ³-H2AX immunofluorescence microscopy. Materials and Methods This study complies with the Declaration of Helsinki and was performed according to local ethics committee approval. Informed patient consent was obtained. Blood samples from 45 patients (13 women, 32 men; mean age, 50.3 years [age range, 20-89 years]) were obtained before and after contrast agent-enhanced cardiac MR imaging. MR imaging-induced double-strand breaks (DSBs) were quantified in isolated blood lymphocytes by using immunofluorescence microscopy after staining the phosphorylated histone variant ³-H2AX. Twenty-nine patients were examined with a myocarditis protocol (group A), 10 patients with a stress-testing protocol (group B), and six patients with flow measurements and angiography (group C). Paired t test was performed to compare excess foci before and after MR imaging. Results The mean baseline DSB level before MR imaging and 5 minutes after MR imaging was, respectively, 0.116 DSB per cell ± 0.019 (standard deviation) and 0.117 DSB per cell ± 0.019 (P = .71). There was also no significant difference in DSBs in these subgroups (group A: DSB per cell before and after MR imaging, respectively, 0.114 and 0.114, P = .91; group B: DSB per cell before and after MR imaging, respectively, 0.123 and 0.124, P = .78; group C: DSB per cell before and after MR imaging, respectively, 0.114 and 0.115, P = .36). Conclusion By using ³-H2AX immunofluorescence microscopy, no DNA DSBs were detected after cardiac MR imaging. (©) RSNA, 2015 Online supplemental material is available for this article.
Findings		No Effects
Status		Completed With Publication
Principal Investigator
Funding Agency		?????
Country		GERMANY
References		Brand, M et al. Radiology., (2015) 277:406-412
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