ID Number		2516
Study Type		In Vitro
Model		Study of genotoxic and cytotoxic endpoints in human lymphocytes exposed in vitro to 7 Tesla MRI signals.
Details		AUTHORS' ABSTRACT: Reddig et al. 2015 (IEEE #6155): The global use of magnetic resonance imaging (MRI) is constantly growing and the field strengths increasing. Yet, only little data about harmful biological effects caused by MRI exposure are available and published research analyzing the impact of MRI on DNA integrity reported controversial results. This in vitro study aimed to investigate the genotoxic and cytotoxic potential of 7 T ultra-high-fieldMRI on isolated human peripheral blood mononuclear cells. Hence, unstimulatedmononuclear blood cells were exposed to 7 T static magnetic field alone or in combination withmaximum permissible imaging gradients and radiofrequency pulses as well as to ionizing radiation during computed tomography and ³-ray exposure. DNA doublestrand breaks were quantified by flow cytometry and automatedmicroscopy analysis of immunofluorescence stained ³H2AX. Cytotoxicity was studied by CellTiter-Blue viability assay and [3H]-thymidine proliferation assay. Exposure of unstimulatedmononuclear blood cells to 7 T static magnetic field alone or combined with varying gradient magnetic fields and pulsed radiofrequency fields did not induce DNA double-strand breaks, whereas irradiation with X- and ³- rays led to a dose-dependent induction of ³H2AX foci. The viability assay revealed a time- and dose-dependent decrease inmetabolic activity only among samples exposed to ³-radiation. Further, there was no evidence for altered proliferation response after cells were exposed to 7 T MRI or low doses of ionizing radiation ( 0.2 Gy). These findings confirm the acceptance of MRI as a safe non-invasive diagnostic imaging tool, but whether MRI can induce other types of DNA lesions or DNA double-strand breaks during altered conditions still needs to be investigated.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		Otto-von-Guericke-Univ Magdeburg, Magdeburg, Germa
Funding Agency		?????
Country		GERMANY
References		Reddig, A et al. PLoS ONE., (2015) 10(7):e0132702.-doi:10.1371/journal.pone.0132702
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