ID Number		2503
Study Type		In Vivo
Model		This study analyzed apoptotic cell formation, using histopathological and immunohistochemical methods (including TUNEL staining) in non-pregnant and pregnant rabbits, and in offspring of the pregnant group exposed to GSM modulated signal at 1800 MHz.
Details		AUTHORS' ABSTRACT: Guler, Seyhan et al. 2011 (IEEE#6083): The purpose of this study was to reveal the apoptotic cell formation, using histopathological and immunohistochemical methods, in non-pregnant and pregnant New Zealand White rabbits, and in offspring of the pregnant group exposed to GSM modulated signal in 1,800 MHz frequency. Apoptotic cells were detected in the brain, eyes, kidneys, liver, lung, heart, and spleen by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) staining. Histopathological changes were observed in the examined organs. TUNEL positivity was seen in the brain (group VI) and eyes (groups IV and VI). In groups I, II, III, and V, the positivity was lesser than 5% and was not taken into account. AUTHORS' ABSTRACT: Guler et al. 2016 (IEEE #6526): Adverse health effects of radiofrequency radiation (RFR) on the ongoing developmental stages of children from conception to childhood are scientifically anticipated subject. This study was performed to identify the effects of global system for mobile communications (GSM) modulated mobile phone like RFR in 1800 MHz frequency on oxidative DNA damage and lipid peroxidation beside the apoptotic cell formation, using histopathological and immunohistochemical methods in the brain tissue of 1-month-old male and female New Zealand White rabbits that were exposed to these fields at their mother's womb and after the birth. Oxidative DNA damage and lipid peroxidation levels were investigated by measuring the 8-hydroxy-22-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels, respectively. Histopathological changes were observed using by hematoxylin and eosin (HE) staining. Apoptotic cells were detected in the examined organs by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. For both male and female infants; 8-OHdG levels increased in the group exposed to RFR in both intrauterine and extrauterine periods compared to the infants that were never exposed to RFR and the ones were exposed when they reached one month of age (p < 0.05). MDA results were different for male and female rabbits. There was no difference between all female infant groups (p > 0.05), while only intrauterine exposure significantly causes MDA level increase for the male infants. HE staining revealed mild lessions in neuronal necrobiosis in brain tissues of female rabbits that had only intaruterine exposure and male rabbits had only extrauterine exposure. Gliosis were mildly positive in brain tissues of rabbits that are exposed only intrauterine period, also the group exposed both intrauterine and extrauterine periods. However, there was no apoptotic change detected by TUNEL staining in the brain tissues of all groups. AUTHORS' ABSTRACT: Meral et al. 2016 (IEEE #6659): BACKGROUND: There have been several Radio Frequency (RF) field researches on various populations and groups of different ages in recent years. However, the most important group for research has been declared as the pregnant women and their babies. OBJECTIVE: The aim of the study was to analyse the effect on apoptotic factors of RF fields on newborn rabbit liver tissues. MATERIALS AND METHODS: Cytochrome c and AIF (Apoptosis Inducing Factor) levels were measured by western blot and caspase 1, 3 and 9 activities were measured by colorimetric method. RESULTS: Cytochrome c and AIF levels were not altered, but all caspase activities were increased in female infant rabbits that exposed to 1800 MHz GSM-like RF signals when they reached 1 month of age and caspase 1 and caspase 3 levels were decreased in male infant rabbits that exposed to 1800 MHz GSM-like RF signals between 15th and 22nd days of the gestational period. Results showed that 1800 MHz GSM-like RF exposure might lead to apoptosis in infant rabbits liver tissues. CONCLUSION: According to the results, we suggest that postnatal RF exposure causes caspase dependent apoptosis in female infant rabbits liver tissues (Tab. 1, Fig. 2, Ref. 27).
Findings		Effects
Status		Completed With Publication
Principal Investigator
Funding Agency		?????
Country		TURKEY
References		Guler, G et al. Bull Vet Inst Pulawy., (2011) 55:127-134 Guler, G et al. Journal of Chemical Neuroanatomy, (2016) 75 (Part B0:128-133 Meral, O et al. Bratisl Med., (2016) 117:672-676
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