ID Number		2476
Study Type		In Vivo
Model		Mice predisposed to Alzheimer's-type disease were exposed to 1950-MHz (5 W/kg)for 2 h/day, 5 days/wk, for 8 months and subjected to behavioral test and histopathology.
Details		AUTHORS' ABSTRACT: Jeong et al. 2015 (IEEE #): The involvement of radiofrequency electromagnetic fields (RF-EMF) in the neurodegenerative disease, especially Alzheimer's disease (AD), has received wide consideration, however, outcomes from several researches have not shown consistency. In this study, we determined whether RF-EMF influenced AD pathology in vivo using Tg-5xFAD mice as a model of AD-like amyloid ² (A²) pathology. The transgenic (Tg)-5xFAD and wild type (WT) mice were chronically exposed to RF-EMF for 8 months (1950 MHz, SAR 5W/kg, 2 hrs/day, 5 days/week). Notably, chronic RFEMF exposure significantly reduced not only A² plaques, APP, and APP carboxyl-terminal fragments (CTFs) in whole brain including hippocampus and entorhinal cortex but also the ratio of A²42 and A²40 peptide in the hippocampus of Tg-5xFAD mice. We also found that parenchymal expression of ²-amyloid precursor protein cleaving enzyme 1(BACE1) and neuroinflammation were inhibited by RF-EMF exposure in Tg-5xFAD. In addition, RF-EMF was shown to rescue memory impairment in Tg-5xFAD. Moreover, gene profiling from microarray data using hippocampus of WT and Tg- 5xFAD following RF-EMF exposure revealed that 5 genes (Tshz2, Gm12695, St3gal1, Isx and Tll1), which are involved in A², are significantly altered inTg-5xFAD mice, exhibiting different responses to RF-EMF in WT or Tg-5xFAD mice; RF-EMF exposure in WT mice showed similar patterns to control Tg-5xFAD mice, however, RF-EMF exposure in Tg- 5xFAD mice showed opposite expression patterns. These findings indicate that chronic RF-EMF exposure directly affects A² pathology in AD but not in normal brain. Therefore, RF-EMF has preventive effects against AD-like pathology in advanced AD mice with a high expression of A², which suggests that RF-EMF can have a beneficial influence on AD. AUTHORS' ABSTRACT: Yeon:ghoon et al. 2016 (IEEE #6429): The increased use of mobile phones has generated public concern about the impact of radiofrequency electromagnetic fields (RF-EMF) on health. In the present study, we investigated whether RF-EMFs induce molecular changes in amyloid precursor protein (APP) processing and amyloid beta (A²)-related memory impairment in the 5xFAD mouse, which is a widely used amyloid animal model. The 5xFAD mice at the age of 1.5 months were assigned to two groups (RF-EMF- and sham-exposed groups, eight mice per group). The RF-EMF group was placed in a reverberation chamber and exposed to 1950 MHz electromagnetic fields for 3 months (SAR 5 W/kg, 2 h/day, 5 days/week). The Y-maze, Morris water maze, and novel object recognition memory test were used to evaluate spatial and non-spatial memory following 3-month RF-EMF exposure. Furthermore, A² deposition and APP and carboxyl-terminal fragment ² (CTF²) levels were evaluated in the hippocampus and cortex of 5xFAD mice, and plasma levels of A² peptides were also investigated. In behavioral tests, mice that were exposed to RF-EMF for 3 months did not exhibit differences in spatial and non-spatial memory compared to the sham-exposed group, and no apparent change was evident in locomotor activity. Consistent with behavioral data, RF-EMF did not alter APP and CTF² levels or A² deposition in the brains of the 5xFAD mice. These findings indicate that 3-month RF-EMF exposure did not affect A²-related memory impairment or A² accumulation in the 5xFAD Alzheimer's disease model.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Korea Inst of Radiological & Med Sci, Seoul
Funding Agency		Ministry of Sci Inst and Future Planning (Korea)
Country		KOREA, REPUBLIC OF
References		Jeong, YJ et al. Curr Alzheimer Res., (2015) 12:481-492 Son, Y et al. Bioelectromagnetics., (2016) 37:391-399 Jeong, YJ et al. Int J Mol Sci., (2018) 19:2103-
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