ID Number		2447
Study Type		In Vitro
Model		The effects of PEMF on hypertrophy and endochondral ossification in rat bone marrow-derived stem cells were assessed by safranin O staining, immunohistochemistry, and quantitative real-time polymerase chain reaction.
Details		AUTHORS' ABSTRACT: Wang et al. 2015 (IEEE #5885): Recapitulation of embryonic endochondral bone formation is a promising alternative approach to bone tissue engineering. However, the time-consuming process is one of the reasons the approach is unpractical. Here, we aimed at accelerating the in vitro endochondral ossification process of tissue engineering by using a pulsed electromagnetic field (PEMF). The rat bone marrow-derived stem cells were chondrogenic or hypertrophic differentiated in a three-dimensional pellet culture system, and treated with different intensities of PEMF (1, 2, and 5 mT with modulation frequency 750 Hz, carrier frequency 75 Hz and a duty ratio of 0.8, 3 h/day for 4 weeks). The effects of PEMF on hypertrophy and endochondral ossification were assessed by safranin O staining, immunohistochemistry, and quantitative real-time polymerase chain reaction. The results suggest that PEMF at 1, 2, and 5 mT may inhibit the maintenance of the cartilaginous phenotype and increase cartilage-specific extracellular matrix degradation in the late stage of chondrogenic differentiation. In addition, among the three different intensities, only PEMF at 1 mT directed the differentiation of chondrogenic-induced stem cell pellets to the hypertrophic stage and promoted osteogenic differentiation. Our findings provide the feasibility to optimize the process of in vitro endochondral ossification with PEMF stimulation.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Sichuan University, Chengdu, China
Funding Agency		?????
Country		CHINA
References		Wang, J et al. Bioelectromagnetics., (2015) 36:35-44
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