ID Number		2374
Study Type		In Vitro
Model		Effect of 1800 MHz on oxidative stress and apoptosis in NIH/3T3 cells.
Details		AUTHORS' ABSTRACT: Hou et al. 2014 (IEEE #5566): To investigate the potential adverse effects of mobile phone radiation, we studied reactive oxygen species (ROS), DNA damage and apoptosis in mouse embryonic fibroblasts (NIH/3T3) after intermittent exposure (5 min on/10 min off, for various durations from 0.5 to 8 h) to an 1800-MHz GSM-talk mode electromagnetic radiation (EMR) at an average specific absorption rate of 2W/kg. A 22,72-dichlorofluorescin diacetate fluorescence probe was used to detect intracellular ROS levels, immunofluorescence was used to detect ³H2AX foci as a marker for DNA damage, and flow cytometry was used to measure apoptosis. Our results showed a significant increase in intracellular ROS levels after EMR exposure and it reached the highest level at an exposure time of 1h (p<0.05) followed by a slight decrease when the exposure continued for as long as 8h. No significant effect on the number of ³H2AX was detected after EMR exposure. The percentage of late-apoptotic cells in the EMR-exposed group was significantly higher than that in the sham-exposed groups (p<0.05). These results indicate that an 1800-MHz EMR enhances ROS formation and promotes apoptosis in NIH/3T3 cells.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Beijing U of Technology, Beijing, China
Funding Agency		?????
Country		CHINA
References		Hou , Q et al. Electromagnetic Biology and Medicine., (2015) 34:85-92
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