ID Number		2178
Study Type		In Vitro
Model		50 Hz exposure to skeletal muscle cells in vitro and analysis of oxidative stress and calcium flux
Details		C2C12 undifferentiated human myoblasts and terminally differentiated myotubes from skeletal muscle were exposed to 50 Hz (ELF) at 0.1 and 1.0 milliT for 30 minutes and evaluated for markers of oxidative stress and calcium flux. The authors report exposure at 1.0 milliT increased reactive oxygen species production in myoblasts and myotubes and decreased mitochondrial membrane potential. Exposure also increased catalase and glutathione peroxidase activity and increased intracellular Ca(2+) levels, enhancing the depolarization response to Ca2+ channel agonists (KCl and caffeine).
Findings		Effects
Status		Completed With Publication
Principal Investigator		University "G. d'Annunzio", Chieti-Pescara, Italy
Funding Agency		Private/Instit.
Country		ITALY
References		Morabito, C et al. Free Radic Biol Med., (2010) 48:579-589
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