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Mouse embryos at the two-cell stage were exposed to calcium ions (0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4 mM) plus electric fields (0.6, 0.8, 1.0, 1.2 and 1.4 kV/cm) and analyzed for blastomere fusion, cleavage, and development of tetraploids to the blastocyst stage. The authors report the process was increased with 1.0 mM calcium plus electrical stimulation at 0.8 kV/cm. |