ID Number		2068
Study Type		In Vivo
Model		900/915 MHz (CW) exposure to rats and analysis of DNA damage in peripheral lymphocytes, brain, liver and kidney.
Details		Wistar rats were exposed to 900 MHz (CW) for 1 hr/day for 2 weeks at 0.6 W/kg (whole body estimate) and DNA in isolated lymphocytes was evaluated using the comet assay. The authors report increased DNA breaks as well as DNA adducts suggesting a mechanism of oxidative stress is likely responsible. AUTHORS' ABSTRACT: Trosic et al. (IEEE #5648): The goal of study was to evaluate DNA damage in rat's renal, liver and brain cells after in vivo exposure to radiofrequency/microwave (Rf/Mw) radiation of cellular phone frequencies range. To determine DNA damage, a single cell gel electrophoresis/comet assay was used. Wistar rats (male, 12 week old, approximate body weight 350 g) (N = 9) were exposed to the carrier frequency of 915 MHz with Global System Mobile signal modulation (GSM), power density of 2.4 W/m2, whole body average specific absorption rate SAR of 0.6 W/kg. The animals were irradiated for one hour/day, seven days/week during two weeks period. The exposure set-up was Gigahertz Transversal Electromagnetic Mode Cell (GTEM--cell). Sham irradiated controls (N = 9) were apart of the study. The body temperature was measured before and after exposure. There were no differences in temperature in between control and treated animals. Comet assay parameters such as the tail length and tail intensity were evaluated. In comparison with tail length in controls (13.5 +/- 0.7 microm), the tail was slightly elongated in brain cells of irradiated animals (14.0 +/- 0.3 microm). The tail length obtained for liver (14.5 +/- 0.3 microm) and kidney (13.9 +/- 0.5 microm) homogenates notably differs in comparison with matched sham controls (13.6 +/- 0.3 microm) and (12.9 +/- 0.9 microm). Differences in tail intensity between control and exposed animals were not significant. The results of this study suggest that, under the experimental conditions applied, repeated 915 MHz irradiation could be a cause of DNA breaks in renal and liver cells, but not affect the cell genome at the higher extent compared to the basal damage.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Inst Med Res & Occup Health, Croatia - vgaraj@imi.hr
Funding Agency		Private/Instit.
Country		CROATIA (local name: Hrvatska)
References		Garaj-Vrhovac, V et al. Toxicol, (2009) 259:107-112 Stewart , A et al. Perspect Public Health., (2012) 132:299-304
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