ID Number		202
Study Type		In Vitro
Model		900 MHz (GSM) exposure to isolated DNA and analysis of strand breaks
Details		Plasmid DNA, isolated from cultures of E.coli bacteria (K12) XL-1Blue transformed with pBluescriptSK) were exposed to 900 MHz (GSM) at SARs of 5.93 mW/kg, 39 mW/kg burst; rectangular wave guide) for 1 to 6 or 18 weeks at 4°C. Attempt to show structural changes of DNA-molecules (relaxed circular and linear DNA-molecules that emerge from single strand breaks or double strand breaks of the originally circular, supercoiled DNA) detected by means of agarose gel electrophoresis. Attempt to show evidence of molecule damages in transformed E.coli-cultures, by comparison of the transformation rates of exposed and unexposed DNA-molecules. No damage of the DNA molecules emerging as single strand breaks or double strand breaks. The ampicillin resistance gene as well as the origin region of the plasmid remained biologically active.
Findings		No Effects
Status		Completed Without Publication
Principal Investigator		University of Bochum, FRG
Funding Agency		FGF, Germany
Country		GERMANY
References
Comments		FGF database ID# H2, FGF contract# 5944