ID Number		1987
Study Type		In Vitro
Model		1-10 MHz ultrasound exposure to HL-60 leukemia cells and analysis of gene expression
Details		HL-60 human promyelocytic leukemia cells were exposed to 1, 3, and 10 MHz ultrasound signals (alone or in combination) for 0 to 20 minutes under different temperature conditions between 30 - 36°C and evaluated for gene expression, either 4 or 24 hours after treatment. The authors report increased Hsp72, Hsp32, and Hsp40 expression with 10 MHz exposure for at least 5 minutes at the higher temperatures. Pulsed exposure patterns (1:2, 5 ms on and 5 ms off) induced Hsp72 to the same level, whereas other pulsed schemes (1:5, 2 ms on and 8 ms off and 1:10; 1 ms on and 9 ms off) had no effect. Sonication of cells for 20 minutes increased Hsp72 expression if it was performed continuously or in 2 x 10 minute periods, but reduced Hsp72 expression when performed for 4 x 5 minute periods. The authors conclude the effects of ultrasound exposure are enhanced by increased culture temperature. In related studies, the authors report on cell survival with ultrasound exposure.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Institut für Biologische Grenzflächen, Karlsruhe G - werner.sontag@ibg.fzk.de
Funding Agency		Private/Instit.
Country		GERMANY
References		Sontag, W et al. Ultrasound Med Biol, (2009) 35:1032-1041 Sontag, W et al. Radiat Environ Biophys, (2009) 48:99-105
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