ID Number		1980
Study Type		In Vitro
Model		1800 MHz (GSM) exposure to HTR-8/SVneo trophoblastic cells and analysis of cell attachment proteins.
Details		HTR-8/SVneo trophoblastic cells were exposed to 1817 MHz (GSM) at 2 W/kg for 1 hour and analyzed for expression of connexins (basement membrane attachment proteins) via rtPCR, Western blot, immunofluorescence, and electron microscopy. The authors report exposure increased Cx40 and Cx43 levels and reduced intercellular gap junctions and Cx protein localization. AUTHORS' ABSTRACT: Cervellati et al. 2013 (IEEE #6279): We investigated the effect of high-frequency electromagnetic fields (HF-EMFs) and 17-²-estradiol on connexins (Cxs), integrins (Ints), and estrogen receptor (ER) expression, as well as on ultrastructure of trophoblast-derived HTR-8/SVneo cells. HF-EMF, 17-²-estradiol, and their combination induced an increase of Cx40 and Cx43 mRNA expression. HF-EMF decreased Int alpha1 and ² 1 mRNA levels but enhanced Int alpha5 mRNA expression. All the Ints mRNA expressions were increased by 17-²-estradiol and exposure to both stimuli. ER-² mRNA was reduced by HF-EMF but augmented by 17-²-estradiol alone or with HF-EMF. ER-² immunofluorescence showed a cytoplasmic localization in sham and HF-EMF exposed cells which became nuclear after treatment with hormone or both stimuli. Electron microscopy evidenced a loss of cellular contact in exposed cells which appeared counteracted by 17-²-estradiol. We demonstrate that 17-²-estradiol modulates Cxs and Ints as well as ER-² expression induced by HF-EMF, suggesting an influence of both stimuli on trophoblast differentiation and migration.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Univ Ferrara, Italy
Funding Agency		Private/Instit.
Country		ITALY
References		Cervellati, F et al. Reprod Toxicol, (2009) 28:59-65 Cervellati, F et al. Oxid Med Cell Longev., (2013) Article ID 280850 :-(11 pages)
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