ID Number		1971
Study Type		In Vitro
Model		Pulsed EMF (PEMF)exposure to human bone marrow mesenchymal stem cells and analysis of growth and differentiation.
Details		Human bone marrow mesenchymal stem cells (BMMSC) were exposed to 50 Hz ELF (PW - 4.5 ms pulse width, 15 Hz pulse rate). The authors report exposure increased the percentage of viable BMMSC cells, resulted in a 20-60% higher cell density during the exponential growth stage, and showed multiple lineage differentiation potential. The authors suggest the increased proliferation rate during exponential phase may have resulted from a decreased lag phase after plating. In a similar study, the authors report exposure as above increased proliferation, expression of osteogenesis related genes (e.g., cbfa1, ALP), and mineralization near the midpoint of osteogenesis. AUTHORS' ABSTRACT: Sun et al. 2010 (IEEE #4691): Osteogenesis is a complex series of events involving the differentiation of mesenchymal stem cells to generate new bone. In this study, we examined the effect of pulsed electromagnetic fields (PEMFs) on cell proliferation, alkaline phosphatase (ALP) activity, mineralization of the extracellular matrix, and gene expression in bone marrow mesenchymal stem cells (BMMSCs) during osteogenic differentiation. Exposure of BMMSCs to PEMFs increased cell proliferation by 29.6% compared to untreated cells at day 1 of differentiation. Semi-quantitative RT-PCR indicated that PEMFs significantly altered temporal expression of osteogenesis-related genes, including a 2.7-fold increase in expression of the key osteogenesis regulatory gene cbfa1, compared to untreated controls. In addition, exposure to PEMFs significantly increased ALP expression during the early stages of osteogenesis and substantially enhanced mineralization near the midpoint of osteogenesis. These results suggest that PEMFs enhance early cell proliferation in BMMSC-mediated osteogenesis, and accelerate the osteogenesis.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Natl Dong Hwa Univ, Hualien, Taiwan
Funding Agency		Private/Instit.
Country		TAIWAN, PROVINCE OF CHINA
References		Sun, LY et al. Bioelectromagnetics., (2010) 31:209-219 Sun, LY et al. Bioelectromag, (2009) 30:251-260
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