ID Number		1959
Study Type		In Vitro
Model		900, 1800 MHz (GSM, CW), 2450 MHz (CW) exposure to human cell lines and analysis of gene expression (hsp70, c-fos, c-jun, CD25, HLA-DR, CD71)
Details		Peripheral lymphocytes from young (22-32 yrs) and old (87-89 yrs) volunteers was isolated, seeded on Petri dishes and treated with 2-deoxy-D-ribose (an apoptosis-inducing agent) and exposed to 1800 MHz GSM 10 minutes on / 20 minutes off, for 44 hours at an SAR of ~1.4 - 2.0 W/kg. No effects of RF exposure were observed on apoptosis, mitochondrial membrane polarization analyses, or hsp70 expression using flow cytometry. In addition, proliferation (assessed by either mitotic index using the cytokinesis blocker cytochalasin-B and by 3H-thymidine incorporation) cell cycle (by flow cytometry using BrdU + PI staining), apoptosis (by reduced PI staining), and mitochondrial activity (using the lipophilic cationic probe JC-1) were all unaffected. A study involving evaluation of CD antigens (CD25, CD95, CD28) on primary isolated T-cells and exposed to 1800 MHz at 2 W/kg showed only a slight (but significant) downregulation of CD95 specifically in CD4+ helper T cells from elderly donors. In a large study funded by the EU 5FP and Reflex in coordination with several labs, the authors reported on exposure of 6 different human cell lines and primary cultures exposed to 900 and 1800 MHz (GSM, CW) and analyzed using Affymetrix gene chips. The authors report no significant changes in NB69 neuroblastoma cells, T lymphocytes, and CHME5 microglial cells. In contrast, EA.hy926 endothelial cells, U937 lymphoblastoma cells, and HL-60 leukemia cells showed between 12 to 34 up- or down-regulated genes. The affected genes did not suggest a stress response but possibly an increase in cellular metabolism. There was no consistent increase in hsp genes or c-fos, c-jun.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Univ. Bologna, Bologna, Italy - bersani@gpxbof.df.unibo.it
Funding Agency		EU, VERUM, Germany, REFLEX, EU 5th Framework, Europe
Country		ITALY
References		Remondini, D et al. Proteomics, (2006) 6:4745-4754 Capri, M et al. Ann N Y Acad Sci, (2006) 1067:493-499
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