ID Number		1870
Study Type		In Vitro
Model		60 Hz ELF exposure to cell lines and evaluation of gene expression using an hsp70-luciferase reporter construct
Details		HeLa (human adenocarcinoma) and BMK16 (baby mouse kidney) cells transfected with a human hsp70 promoter-luciferase reporter construct were exposed to either conventional heating (43oC for 20 minutes in a water bath, followed by 30 minutes at 37oC), a 60 Hz ELF magnetic field (8 or 80 microTesla) for 20 minutes in a 37oC incubator using a helmholtz coil, or conventional heating followed by 60 Hz ELF exposure. ELF exposure increased hsp70 gene expression in a dose dependent manner, and synergized with conventional heating (luciferase activity increased 16 fold over conventional heating or magnetic field exposure alone). The authors cite literature contradicting their results (Bernardini et al 2007; Coulton et al 2004; Czyz et al 2004), but conclude the effects are real and that their data confirms prior reports from the laboratory of Reba Goodman that the HSP70 promoter has active electromagnetic field responsive elements (EMRE).
Findings		Effects
Status		Completed With Publication
Principal Investigator		Universidad Autonoma de Nuevo Leon (UANL), Mexico
Funding Agency		Private/Instit.
Country		MEXICO
References		Rodriguez de la Fuen, AO et al. Cell Biol Int, (2009) 33:419-423
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