ID Number		1801
Study Type		In Vitro
Model		2450 MHz (AM 50 Hz)exposure to isolated protein and evaluation of conformational and activity changes
Details		Porcine heart protein citrate synthase (which unfolds and binds strongly to the chaperone alpha crystallin when exposed to increased temperature) in solution was exposed to 2450 MHz RF for 10, 15, or 20 seconds in a conventional microwave oven at a reported SAR of 4.85 kW/kg. Temperature rise was determined with a luxtron probe positioned near the bottom of the tube. The amount of unfolded CS was determined by affinity capture on an alpha crystallin substrate. The authors report heating by microwave resulted in higher CS unfolding and different kinetics than conventional heating in a water bath. The authors do not think the result is an effect of hot spots, and suggest it is a non-thermal contribution of microwave energy.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		University of Sydney, Australia
Funding Agency		Private/Instit.
Country		AUSTRALIA
References		George, DF et al. Bioelectromagnetics, (2008) 29:324-330
Comments		No description of how SAR was determined - simply a rack in a microwave oven