ID Number		1750
Study Type		In Vitro
Model		900 MHz (GSM) exposure to human neuroblastoma cells and analysis of gene expression
Details		Human neuroblastoma cells (SH-SY5Y) were exposed to 900 MHz (GSM) at 1 W/kg for 5, 15, 30 minutes or 6 or 24 hours and evaluated for gene expression using rtPCR and western blot. The authors report an increase in Egr-1 expression and protein with a max increase at 30 minutes of exposure, followed by a decrease to baseline by 24 hours. In addition, the authors report increases in downstream regulatory element Elk-1 expression and SAPK/JNK kinase activity, and decreased Bcl-2 and survivin expression. No early changes were detected in cell viability (via MTT assay), but after 24 hours of exposure viability significantly decreased and was paralleled by a G2-M cell cycle arrest and onset of apoptosis.
Findings		Effects
Status		Completed With Publication
Principal Investigator		University of Bari, Italy
Funding Agency		Private/Instit.
Country		ITALY
References		Buttiglione, M et al. J Cell Physiol, (2007) 213:759-767
Comments		Erk-1 (extracellular-signal-regulated kinase) is MAPK with upstream regulation by Ras (G-protein), Raf (MAPKKK),and MEK (MAPKK). The MAPK/Erk-1 cascade (which also includes downstream activation of Egr-1) seems to control such fundamental cellular processes as proliferation, differentiation, survival, and apoptosis and may be instrumental in the biochemical pathways linked to learning and neuronal development (see Friedman et al 2007)