ID Number		1568
Study Type		In Vitro
Model		2.45 GHz (CW) and 1.5 GHz (PDC) exposure to C3H10T-1/2 cells (analysis of transformation) and human glial cells (gene expression).
Details		C3H 10T1/2 cells were exposed to 2450 MHz (CW, PW) RF at high SARs (from 5 W/kg up to 200 W/kg) for 2 hours in a circular waveguide +/- co-treatment with 20-Methylcholanthrene. 12-O-tetra-decanoylphorbol-13- acetate (TPA) was used as a positive control for transformation. The authors report no effects of RF alone at SARs up to 200 W/kg, and no effect of RF + MC at SARs up to 100 W/kg. At 200 W/kg, RF did synergize with MC as well as MC + TPA, but this could well have been due to local heating effects. The authors suggest that RF exposure is not a direct carcinogen, but could act as a co-carcinogen at SAR levels of 100 W/kg or greater. AUTHORS' ABSTRACT: Sakurai et al. 2011 (IEEE #5668): The increasing use of mobile phones has aroused public concern regarding the potential health risks of radiofrequency (RF) fields. We investigated the effects of exposure to RF fields (2.45 GHz, continuous wave) at specific absorption rate (SAR) of 1, 5, and 10 W/kg for 1, 4, and 24 h on gene expression in a normal human glial cell line, SVGp12, using DNA microarray. Microarray analysis revealed 23 assigned gene spots and 5 non-assigned gene spots as prospective altered gene spots. Twenty-two genes out of the 23 assigned gene spots were further analyzed by reverse transcription-polymerase chain reaction to validate the results of microarray, and no significant alterations in gene expression were observed. Under the experimental conditions used in this study, we found no evidence that exposure to RF fields affected gene expression in SVGp12 cells.
Findings		Effects (only at thermal levels)
Status		Completed With Publication
Principal Investigator		Tokyo Metropolitan University - miyakosh@cc.hirosaki-u.ac.jp
Funding Agency		MIC, Japan
Country		JAPAN
References		Wang, J et al. J Radiat Res (Tokyo), (2005) 46:351-361 Sakurai, T et al. J Radiat Res., (2011) 52:185-192
Comments