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(Database last updated on Mar 27, 2024)
| ID Number | 1543 | |
| Study Type | In Vitro | |
| Model | In vitro studies at 1800 MHz (GSM, CW) and 30 GHz (CW) on a) MCF-7 cells and primary rat neurons and analysis of gene and protein expression and b) and membrane receptors in human amniotic cells. | |
| Details | In initial studies, MCF-7 cells exposed to 1800 MHz (GSM, CW) RF at SARs of 0.1 to 4 W/kg from a waveguide, temperature maintained within +/- 0.5 degrees C. The authors describe inconsistent changes in (as yet unidentified) protein expression as visualized on 2-D gels following exposure at 2 or 3.5 W/kg for 1, 3, 6, 12, and 24 hours, although the authors concluded weak effects on protein expression due to exposure. A recent study in Proteomics (2006), however, was not able to replicate these results using Affymetrix chips or other assays such as rtPCR. The authors suggest the initial findings were normal variations in the assays. Similar gene chip studies using primary neurons isolated from newborn Sprague Dawley rats and exposed 24 hrs at 2 W/kg resulted in expression increases in 24 and decreases in 10 genes using gene chip analysis and confirmation with rtPCR, many involved in regulation of ion channels, transcription, signal transduction, cytoskeleton elements, and mobility. Among the genes was Mbp and Plp (up-regulated) and Egr-1 (down-regulated). The authors reported increased clustering of Egr-1 receptor following a 15 minute exposure to GSM RF (but not CW) at 0.5, 1, 2, and 4 W/kg, and that noise due to simultaneous exposure to 50 Hz ELF at 2 uT blocked the clustering effect. Exposure at 1800 MHz (GSM) for 1 hour at 3 W/kg also increased phosphorylation of H2AX suggesting increased DNA damage or genomic instability. In studies exposing HaCaT keratinocytes to 30.16 GHz millimeter waves at 1 or 3.5 mW/cm2, the authors report increases in PAR-2, ERGIC-53, and ERGIC-53 genes. AUTHORS' ABSTRACT: Sun et al. 2012 (IEEE #6227): PURPOSE: Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and phosphorylation in human amniotic (FL) cells. MATERIALS AND METHODS: Receptor clustering on cellular membrane surface was analyzed using immunofluorescence assessed by confocal microscopy, and phosphorylation of EGF receptors was measured by western blot technology. EGF treatment served as a positive control. RESULTS: The results showed that, compared with sham exposure, exposure to RFR at specific absorption rate (SAR) of 0.5, 1.0, 2.0, or 4.0 W/kg for 15 min significantly induced EGF receptor clustering and enhanced phosphorylation on the tyrosine-1173 residue in FL cells, whereas exposure to a SAR 0.1 W/kg radiation for 15 min did not cause a significant effect. CONCLUSION: Based on the results of this experiment, we conclude that membrane receptors could be one of the main targets that RFR interacts with cells, and the dose-rate threshold, in the case of EGF receptors, is between SAR of 0.1 and 0.5 W/kg. The results indicate a sigmoid dependence of RFR effects on intensity. |
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| Findings | Effects | |
| Status | Completed With Publication | |
| Principal Investigator | Zhejiang University, Hangzhou, Zhejiang, China - hch@mail.hz.zj.cn | |
| Funding Agency | CDC / NIEHS, China | |
| Country | CHINA | |
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