ID Number		125
Study Type		In Vitro
Model		835, 847 MHz (CDMA, CW) exposure to cell lines and analysis of heat shock response
Details		HA-1 Chinese hamster cells, PEER cells, and C3H 10T1/2 fibroblast cells were exposed to 835.62 MHz (FM) and 847.74 MHz (CDMA) at 0.6 or 5 W/kg for between 2 hours and 7 days in a radial transmission line (RTL) exposure system. Heat shock factor 1 (HSF-1) was measured by gel shift assay as an indicator of the first step in the induction of the stress response. As a positive control, increases in HSF-1 binding could be seen with non-RF temperature shifts as small as 1-2oC for 15 minutes. No effects of RF exposure were observed when exposures were carried out at the levels emitted by a mobile phone transmitting at maximal power, or at higher levels while maintaining the cells at 37oC. In a study performed as a replication / validation of Leszczynski et al (Differentiation (2002) 70:120-127), the authors report no effects of 847 MHz (TDMA) at 5 W/kg for 1, 2, or 24 hours, or 900 MHz (GSM) at 3.7 W/kg for 1, 2, or 5 hours on hsp27 phosphorylation levels using 2-D gels. Increased temperature (41 and 45 degrees) lead to a dose dependent increase in hsp27 phosphorylation.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		Washington University, USA - rotiroti@radonc.wustl.edu
Funding Agency		Motorola, MMF, GSM Association
Country		UNITED STATES
References		Vanderwaal, VMM et al. Int J Hyperthermia, (2006) 22:507-519 Laszlo, A et al. Radiation Research, (2005) 164:163-172
Comments