ID Number		124
Study Type		In Vitro
Model		835, 847 MHz (CDMA, CW) exposure to cell lines and analysis of oxidative stress parameters
Details		The J774.16 mouse macrophage cell line was exposed to 835 (FM) & 847 MHz (CDMA) for 20 - 22 hours at 0.6 W/kg in a radial transmission line (RTL) system following stimulation with bacterial LPS and IFN. Levels of nitric oxide and hydroperoxide production (a measure of intracellular oxidants), oxidized glutathione (an indirect measure of oxidative damage), Mn superoxide dismutase, CuZn superoxide dismutase, catalase, glutathione peroxidase, and total glutathione content (a measure of antioxidant defenses) were measured, as well as trypan blue dye exclusion (a measure of cell cytotoxicity). No effect of RF on oxidative parameters or cell viability was reported.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		Washington University, USA - rotiroti@radonc.wustl.edu
Funding Agency		Motorola
Country		UNITED STATES
References		Hook, GJ et al. Radiation Research, (2004) 162:497-504
Comments		In one set of initial experiments, RF exposure resulted in initial decreases in Mn-SOD activity, a statistically significant decrease in viability, and an increase in oxidized glutathione. However, these observations were not consistently repeated in further experiments. An initial follow-up to clarify if the initial findings showed a dose response was not completed as the original findings could not be replicated by the lab.