ID Number		1194
Study Type		In Vitro
Model		ELF exposure to primary gonad cell cultures and analysis of gene expression and localization of adhering / attachment / structural proteins
Details		Mouse primary testicular interstitial (Leydig) or human ovarian granulosa cells were exposed to a sinusoidal 50 (or 16) Hz magnetic field at 100 uT for 48 hr in a CO2 incubator. Non-exposed, human choriogonadotropin stimulated and toxic metal ion (Ni2+) exposed cells were also cultured as positive controls. Following exposure, the localization of cadherins, ²-catenin and tubulin was immunocytochemical detected and examined by fluorescent microscopy. The determination of the amount of adherens cell contact proteins in Leydig cells by Western blot is in progress. Start date December 2001, end date December 2004.
Findings		Ongoing
Status		Ongoing
Start Date
End Date
Principal Investigator		National Research Institute for Radiobiology and R - somosy@hp.osski.hu
Funding Agency		Ministry of Welfare, Hungary
Country		HUNGARY
References		Forgacs, Z et al. Acta Biologica Szegediensis , (2003) 47:27-30
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