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(Database last updated on Mar 27, 2024)
| ID Number | 1176 | |
| Study Type | Human / Provocation | |
| Model | 50 Hz exposure from high-voltage electric injury and tissue necrosis and thrombosis of blood vessels | |
| Details | Patients (n=3) experiencing high-voltage electric injury (50 Hz) examined for tissue necrosis and thrombosis of blood vessels, both proximal and remote from entry and exit site of electrical current. Plasma levels of TAT, F(1+2), PAI-1, and t-PA were determined 96 hours after trauma. Haemostasis related to endothelial cell damage was assessed in vitro 72 hours after exposure to 50 Hz for 1, 10, 30, and 50 electric field periods (duration of 20 ms) at a field strength of 60 V/cm and evaluation of cell culture supernatant for protein S, TF, ET-1, PGI(2), NO, t-PA, and PAI-1 and thrombo-modulin expression by immuno-histochemistry. Clotting activation was observed in patients by increased levels of F(1+2) and TAT between 12 and 72 hours after injury. Fibrinolysis was disturbed as evidenced by high PAI-1. One patient presented thrombosis of vessels by day 3. In vitro, PAI-1 increased significantly (p<0.05) in medium of cells with an application of 30 and 50 periods between 2 and 48 hours. Between 4 and 72 hours, the concentration of t-PA was significantly lower (p<0.05) in the medium of HUVECs exposed to 10, 30, and 50 periods, whereas there was a significant increase (p<0.05) in the concentration of TF in the cell groups with an application of 30 and 50 periods. 24, 48, and 72 hours after injury, there was weak or no staining for thrombomodulin in HUVECs with an application of 30 and 50 periods. The disturbed balance between clotting system and fibrinolysis seen in vitro after electric injury might explain the clinical observation of a progressive thrombosis of blood vessels after electric injury leading to tissue loss. |
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| Findings | Effects | |
| Status | Completed With Publication | |
| Principal Investigator | FEMU- RWTH Aachen FRG - info@femu.rwth-aachen.de | |
| Funding Agency | Aachen Univ Hospital, Germany | |
| Country | GERMANY | |
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