ID Number		1093
Study Type		In Vitro
Model		900 (CW, GSM) exposure to cell lines and yeast and analysis of growth, apoptosis, and oxidative stress (MX-initiated assay)
Details		L929 fibrosarcoma cell lines were exposed to 900 MHz (GSM or CW) RF at either 0.3 or 1 W/kg in combination with the herbicide Vinclozolin or MX (3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)- furanone) at different concentrations and different treatment durations. The exposure durations were 10 or 30 minutes for oxidative stress and between 30 minutes and 2 hours for the other biological endpoints. Reactive oxygen species evaluation was performed 10, 20, 40, or 60 minutes following exposure. Cell analysis includes oxidative stress (measurement of the activity of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose-6 phosphate dehydrogenase, and the intracellular concentration of glutathione, and reactive oxygen species), cell proliferation, cell cycle, apoptosis, mitochondrial membrane potential modifications and expression of selected proto-oncogenes, such as c-jun, c-fos and c-myc. The authors report no statistically significant effects from RF exposure.
Findings		No Effects
Status		Completed With Publication
Principal Investigator		ICEmB, CNR-IREA, Univ Naples, Italy - Scarfi.mr@irea.cnr.it
Funding Agency		EU, CEMFEC, EU 5th Framework, Europe, CNR-IREA
Country		ITALY
References		Zeni, O et al. Radiation Research, (2007) 167:306-311
Comments		This study belongs to the consortium CEMFEC funded by EU Fifth Framework programme "Quality of Life and Management of Living Resources"