ID Number		109
Study Type		In Vitro
Model		27 & 2450 MHz (CW & PW-20 Hz) exposure to cell lines and analysis of growth
Details		LN71 (human glioma), CHO (chinese hamster ovary), and human primary lymphocyte cells were exposed to 27 MHz (CW) MW in a coaxial exposure system (Guy, 1977, Radio Science 12:87-96) or 2450 MHz (CW or PW- 50 Hz pulse repetition rate, 6.67-msec pulse duration) in a waveguide system (Cleary et al., 1985, Bioelectromagnetics 6:53-60; Liu and Cleary, 1988, Bioelectromagnetics 9:249-257) for 2 hr at SARs of 0-200 W/kg during G0/G1, S, or G2/M phases of the cell cycle. Both 27 and 2450 MHz exposure systems were characterized as “isothermal”. After exposure, cells were removed from the exposure apparatus and incubated for 1, 3, or 5 days and assayed for proliferation (DNA synthesis) and RNA synthesis by incorporation of 3H-thymidine (3H-TdR) and 3H-uridine (3H-UdR), respectively. Exposure at or below 50 W/kg increased 3H-TdR (proliferation rate) and 3H-UdR (endogenous transcription), while exposure to at higher SAR decreased proliferation (3H incorporation. MW induced proliferation of CTLL-2 cells was dependent upon IL-2. The authors speculate that the results suggest MW exposure alters membrane signal transduction, a direct effect that is independent of heating, and that this effect depends on the presence during exposure of unoccupied high-affinity IL-2 receptors. No effect on cell viability or morphology was observed.
Findings		Effects
Status		Completed With Publication
Principal Investigator		Medical College of Virginia, USA
Funding Agency		Private/Instit.
Country		UNITED STATES
References		Cleary, SF et al. FASEB J., (1996) 10:913-919 Cleary, SF et al. Bioelectrochem. & Bioenerg., (1996) 39:167-173 Cao, G et al. Bioelectrochem. Bioenerg., (1995) 37:140-1995 Cleary, SF et al. Bioelectromagnetics, (1990) 11:47-56 Cleary, SF et al. Radiat. Res., (1990) 121:38-45
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